Switches.ELM

Switch #:

SWTI000606

Switch type:
Binary

Switch subtype:
Physicochemical compatibility

Description:
Phosphorylation of S645 in Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma (Pip5k1c) impedes binding to AP-2 complex subunit beta (Ap2b1), while dephosphorylation by calcineurin promotes binding. These phosphorylation and dephosphorylation events are important for the regulation of clathrin coat formation associated with synaptic vesicles.

Participants:
(1) Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma (Pip5k1c)
(2) AP-2 complex subunit beta (Ap2b1)

Interactions

Interaction #1 Pip5k1c - Ap2b1

Interfaces
(1) ELM:TRG_AP2beta_CARGO_2 motif (₆₃₃IYFPTDERSWVY₆₄₄) in Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma (Pip5k1c)
(2) Beta2-adaptin appendage, C-terminal sub-domain_(826-936) in AP-2 complex subunit beta (Ap2b1)

Interaction Regulation
PTM-dependent Inhibition (Phosphorylation of S₆₄₅ on Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma (Pip5k1c)) of the Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma (Pip5k1c) TRG_AP2beta_CARGO_2 motif - AP-2 complex subunit beta (Ap2b1) Beta2-adaptin appendage, C-terminal sub-domain interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: S₆₄₅: Cyclin-dependent kinase 5 (Cdk5)

Context

Switch pathways

References

(1) Clathrin regulates the association of PIPKIgamma661 with the AP-2 adaptor beta2 appendage.
Thieman et al. J. Biol. Chem. (2009)