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Switch #:
SWTI000204
Switch type:
Binary
Switch subtype:
Physicochemical compatibility

Description:
Phosphorylation of several serine residues surrounding the EB1-binding motifs of CLIP-associating protein 2 (CLASP2) by Glycogen synthase kinase-3 beta (GSK3B) and Glycogen synthase kinase-3 beta (GSK3B) and Glycogen synthase kinase-3 beta (GSK3B) and Glycogen synthase kinase-3 beta (GSK3B) and Glycogen synthase kinase-3 beta (GSK3B) inhibits its interaction with Microtubule-associated protein RP/EB family member 1 (MAPRE1).

Participants:
(1) CLIP-associating protein 2 (CLASP2)
(2) Microtubule-associated protein RP/EB family member 1 (MAPRE1)

Interactions
Interaction #1 CLASP2 - MAPRE1

Interfaces
(1) LIG_SxIP_EBH_1 motif (515RSSRIPRPSVS525) in CLIP-associating protein 2 (CLASP2)
(2) EB1-like C-terminal motif (209-248) in Microtubule-associated protein RP/EB family member 1 (MAPRE1)

Interaction Regulation
PTM-dependent Abrogation (Phosphorylation of S525, S529, S533, S537 and S541 on CLIP-associating protein 2 (CLASP2)) of the CLIP-associating protein 2 (CLASP2) LIG_SxIP_EBH_1 motif - Microtubule-associated protein RP/EB family member 1 (MAPRE1) EB1-like C-terminal motif interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: S525: Glycogen synthase kinase-3 beta (GSK3B); S529: Glycogen synthase kinase-3 beta (GSK3B); S533: Glycogen synthase kinase-3 beta (GSK3B); S537: Glycogen synthase kinase-3 beta (GSK3B); S541: Glycogen synthase kinase-3 beta (GSK3B)

Inferred Regulatory Enzymes for switch
Putative modifying enzymes for residue: S541 : Cyclin-dependent kinase 1 (CDK1).

Context
Switch localisation
Curated: cytosol
ELM curated: cytosol, microtubule
Inferred: Golgi apparatus, cytosol, cytoplasmic microtubule

Switch cell cycle phase
Curated: mitotic prometaphase

Switch pathways
Curated: Kinetochore capture of astral microtubules
References

(1) GSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment.
Kumar et al. J. Cell Biol. (2009)

(2) Spatial regulation of CLASP affinity for microtubules by Rac1 and GSK3beta in migrating epithelial cells.
Wittmann et al. J. Cell Biol. (2005)

See also

Other switches involving participants
Microtubule-associated protein RP/EB family member 1 (MAPRE1) - 2 more (view)

Other switches involving interfaces
LIG_SxIP_EBH_1 - 2 more (view)
EB1-like C-terminal motif - 2 more (view)


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