Switches.ELM

Switch #:

SWTI000205

Switch type:
Binary

Switch subtype:
Physicochemical compatibility

Description:
Phosphorylation of S403 in the RhoGAP-binding motif of DCC-interacting protein 13-alpha (APPL1), possibly by PKA, inhibits its interaction with Inositol polyphosphate 5-phosphatase OCRL-1 (OCRL).

Participants:
(1) DCC-interacting protein 13-alpha (APPL1)
(2) Inositol polyphosphate 5-phosphatase OCRL-1 (OCRL)

Interactions

Interaction #1 APPL1 - OCRL

Interfaces
(1) ELM:LIG_RhoGAP_OCRL_1 motif (₄₀₃SFQQRHESLRPAA₄₁₅) in DCC-interacting protein 13-alpha (APPL1)
(2) RhoGAP domain_(735-878) in Inositol polyphosphate 5-phosphatase OCRL-1 (OCRL)

Interaction Regulation
PTM-dependent Abrogation (Phosphorylation of S₄₀₃ on DCC-interacting protein 13-alpha (APPL1)) of the DCC-interacting protein 13-alpha (APPL1) LIG_RhoGAP_OCRL_1 motif - Inositol polyphosphate 5-phosphatase OCRL-1 (OCRL) RhoGAP domain interaction

Additional Information
Affinity : S403: 43.1 µM, pS403: N.D. µM

Context

Switch localisation

References

(1) A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway.
Erdmann et al. Dev. Cell (2007)

(2) Recognition of the F&H motif by the Lowe syndrome protein OCRL.
Pirruccello et al. Nat. Struct. Mol. Biol. (2011)