Switches.ELM

Switch #:

SWTI000276

Switch type:
Cumulative

Switch subtype:
Rheostatic

Description:
Phosphorylation of S2789 and S2793 adjacent to the EBH-binding motif of Adenomatous polyposis coli protein (APC), by , respectively, gradually reduces the affinity of its interaction with Microtubule-associated protein RP/EB family member 1 (MAPRE1).

Participants:
(1) Adenomatous polyposis coli protein (APC)
(2) Microtubule-associated protein RP/EB family member 1 (MAPRE1)

Interactions

Interaction #1 APC - MAPRE1

Interfaces
(1) LIG_SxIP_EBH_1 motif (₂₈₀₁RPSQIPTPVNN₂₈₁₁) in Adenomatous polyposis coli protein (APC)
(2) EB1-like C-terminal motif_(209-248) in Microtubule-associated protein RP/EB family member 1 (MAPRE1)

Interaction Regulation
PTM-dependent Inhibition (Phosphorylation of S₂₇₈₉ and S₂₇₉₃ on Adenomatous polyposis coli protein (APC)) of the Adenomatous polyposis coli protein (APC) LIG_SxIP_EBH_1 motif - Microtubule-associated protein RP/EB family member 1 (MAPRE1) EB1-like C-terminal motif interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: S₂₇₈₉: Cyclin-dependent kinase 1 (CDK1); S₂₇₉₃: GSK-3 subfamily

Additional Information
Affinity : S2789-S2793: 5.8 µM, pS2789-S2793: 17.9 µM, S2789-pS2793: 32 µM

Context

References

(1) Structural insights into the EB1-APC interaction.
Honnappa et al. EMBO J. (2005)

(2) An EB1-binding motif acts as a microtubule tip localization signal.
Honnappa et al. Cell (2009)