Switches.ELM

Switch #:

SWTI000328

Switch type:
Specificity

Switch subtype:
Domain hiding

Description:
An intramolecular interaction of a Bin1 SH3 binding motif, encoded by exon 10, with the Dynamin-2 (DNM2) SH3 domain prevents binding of dynamin2 to the Bin1 SH3 domain. Binding of PI(4,5)P2 to the overlapping PI(4,5)P2 binding motif encoded by exon 10 relieves the intramolecular auto-inhibitory interaction and allows the Bin1 SH3 domain to interact with the dynamin2 PxxP motif

Participants:
(1) Isoform BIN1 of Myc box-dependent-interacting protein 1 (BIN1)
(2) Dynamin-2 (DNM2)

Interactions

Interaction #1 BIN1 - BIN1(intramolecular)
intramolecular
Is mutually exclusive with Interaction #2 DNM2 - BIN1

Interfaces
(1) ELM:LIG_SH3_8 motif (₂₆₅RRKK₂₆₈) in Isoform BIN1 of Myc box-dependent-interacting protein 1 (BIN1)
(2) Variant SH3 domain_(385-451) in Isoform BIN1 of Myc box-dependent-interacting protein 1 (BIN1)

Interaction #2 DNM2 - BIN1

Is mutually exclusive with Interaction #1 BIN1 - BIN1

Interfaces
(3) LIG_SH3_2 motif (₈₂₉PQIPSR₈₃₄) in Dynamin-2 (DNM2)
(4) Variant SH3 domain_(385-451) in Isoform BIN1 of Myc box-dependent-interacting protein 1 (BIN1)

Interaction Regulation
Effector binding Abrogation (Isoform BIN1 of Myc box-dependent-interacting protein 1 (BIN1)) of the Dynamin-2 (DNM2) LIG_SH3_2 motif - Isoform BIN1 of Myc box-dependent-interacting protein 1 (BIN1) Variant SH3 domain interaction

Context

Switch localisation
ELM curated: cytosol, focal adhesion, plasma membrane

Inferred: nucleus

References

(1) Regulation of Bin1 SH3 domain binding by phosphoinositides.
Kojima et al. EMBO J. (2004)