Switches.ELM

Switch #:

SWTI000332

Switch type:
Specificity

Switch subtype:
Domain hiding

Description:
The switch from cytokinesis to intracellular bridge formation in differentiating male germ cells is mediated by Testis-expressed protein 14 (Tex14). Binding of Tex14 to Centrosomal protein of 55 kDa (Cep55) prevents binding of ALIX (Programmed cell death 6-interacting protein (Pdcd6ip)) and Tumor susceptibility gene 101 protein (Tsg101) to Cep55, which is required for abscission at the end of cytokinesis. Tex14 uses the same site on Cep55 as ALIX (Pdcd6ip) and Tsg101. The strong interaction between Tex14 and Cep55 together with the avidity effect that results from interaction of MKLP1 with both Tex14 and Cep55 prevent recruitment of ALIX (Pdcd6ip) and Tsg101 for abscission.

Participants:
(1) Testis-expressed protein 14 (Tex14)
(2) Centrosomal protein of 55 kDa (Cep55)
(3) Programmed cell death 6-interacting protein (Pdcd6ip)

Interactions

Interaction #1 Tex14 - Cep55

Is mutually exclusive with Interaction #2 Pdcd6ip - Cep55

Interfaces
(1) LIG_EABR_CEP55_1 motif (₇₉₁GPPSLAY₇₉₇) in Testis-expressed protein 14 (Tex14)
(2) TSG101 and ALIX binding domain of CEP55_(171-205) in Centrosomal protein of 55 kDa (Cep55)

Interaction #2 Pdcd6ip - Cep55

Is mutually exclusive with Interaction #1 Tex14 - Cep55

Interfaces
(3) LIG_EABR_CEP55_1 motif (₈₀₁GPPYPTY₈₀₇) in Programmed cell death 6-interacting protein (Pdcd6ip)
(4) TSG101 and ALIX binding domain of CEP55_(171-205) in Centrosomal protein of 55 kDa (Cep55)

Interaction Regulation
Effector binding Abrogation (Testis-expressed protein 14 (Tex14)) of the Programmed cell death 6-interacting protein (Pdcd6ip) LIG_EABR_CEP55_1 motif - Centrosomal protein of 55 kDa (Cep55) TSG101 and ALIX binding domain of CEP55 interaction

Additional Information
Structural information: 3E1R

Context

Switch localisation

References

(1) TEX14 interacts with CEP55 to block cell abscission.
Iwamori et al. Mol. Cell. Biol. (2010)