Switch #: SWTI000353

Switch type: Avidity-sensing

Description:
Phosphorylation of two 14-3-3-binding motifs in Protein kinase C epsilon type (PRKCE) induces high-avidity binding to dimeric 14-3-3 protein zeta/delta (YWHAZ).

Participants:
(1) 14-3-3 protein zeta/delta (YWHAZ)
(2) Protein kinase C epsilon type (PRKCE)

This is a sequential switch. The interactions in this switch take place in a defined order. The order is defined by numbers to the left of each interaction. More than one interaction can occur in each step, these interactions occur independently but are necessary for future step

1Interaction #1 YWHAZ - YWHAZ

Interfaces
(1) 14-3-3 protein in 14-3-3 protein zeta/delta (YWHAZ)
(2) 14-3-3 protein _(3-236) in 14-3-3 protein zeta/delta (YWHAZ)
Additional Information
Structural information: 2WH0

2Interaction #2 PRKCE - Interaction #1 YWHAZ - YWHAZ

Requires Interaction #1 YWHAZ - YWHAZ

Interfaces
(3) LIG_14-3-3_3 motif (₃₄₃RSKSAP₃₄₈) in Protein kinase C epsilon type (PRKCE)
(4) Interaction #1 YWHAZ - YWHAZ

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S₃₄₆ on Protein kinase C epsilon type (PRKCE)) of the Protein kinase C epsilon type (PRKCE) LIG_14-3-3_3 motif - 14-3-3 protein interaction

Additional Information
Affinity : pS346-S368: 9.8 µM, S346-pS368: >100 µM, pS346-pS368: 0.059 µM
Structural information: 2WH0

2Interaction #3 PRKCE - Interaction #1 YWHAZ - YWHAZ

Requires Interaction #1 YWHAZ - YWHAZ

Interfaces
(5) LIG_14-3-3_3 motif (₃₆₅KALSFD₃₇₀) in Protein kinase C epsilon type (PRKCE)
(6) Interaction #1 YWHAZ - YWHAZ

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S₃₆₈ on Protein kinase C epsilon type (PRKCE)) of the Protein kinase C epsilon type (PRKCE) LIG_14-3-3_3 motif - 14-3-3 protein interaction

Inferred Regulatory Enzymes for switch
Putative modifying enzymes for residue: S₃₆₈ : Protein kinase C alpha type (PRKCA), Protein kinase C epsilon type (PRKCE).

Additional Information
Affinity : pS346-S368: 9.8 µM, S346-pS368: >100 µM, pS346-pS368: 0.059 µM
Structural information: 2WH0

(1) Recognition of an intra-chain tandem 14-3-3 binding site within PKCepsilon.
Kostelecky et al. EMBO Rep. (2009)

Other switches involving participants
14-3-3 protein zeta/delta (YWHAZ) - 11 more (view)

Other switches involving interfaces
14-3-3 protein - 38 more (view)
LIG_14-3-3_3 - 16 more (view)