Switches.ELM

Switch #:

SWTI000628

Switch type:
Binary

Description:
Alternative splicing removes the PDZ-binding motif of Plasma membrane calcium-transporting ATPase 4 (ATP2B4), abrogating binding to Disks large homolog 1 (DLG1). A much lower affinity was recorded for the third PDZ domain of DLG1 (in in the micromolar range (KD = 1.2 microM) compared to nanomolar affinity (KD = 1.6 nM)). PMCA4b and DLG1 are co-expressed in kidney and intestinal epithelial cells as well as in several areas of the brain.

Participants:
(1) Plasma membrane calcium-transporting ATPase 4 (ATP2B4)
(2) Disks large homolog 1 (DLG1)

Interactions

Interaction #1 ATP2B4 - DLG1

Interfaces
(1) LIG_PDZ_Class_1 motif (₁₂₃₆SLETSV₁₂₄₁) in Plasma membrane calcium-transporting ATPase 4 (ATP2B4)
(2) PDZ domain (Also known as DHR or GLGF)_(319-405) in Disks large homolog 1 (DLG1)

Interaction Regulation
Alternative splicing Abrogation of the Plasma membrane calcium-transporting ATPase 4 (ATP2B4) LIG_PDZ_Class_1 motif - Disks large homolog 1 (DLG1) PDZ domain (Also known as DHR or GLGF) interaction

Additional Information
Affinity : hDLG PDZ1/2: 0.0016 µM, hDLG PDZ3: 1.2 µM

Context

Switch localisation
ELM curated: cytosol, internal side of plasma membrane

References

(1) Plasma membrane Ca2+ ATPase isoform 4b binds to membrane-associated guanylate kinase (MAGUK) proteins via their PDZ (PSD-95/Dlg/ZO-1) domains.
Kim et al. J. Biol. Chem. (1998)