Switches.ELM

Switch #:

SWTI000666

Switch subtype:
Uncategorised

Description:
Sumoylation of K160 induces binding to the Protein PML (PML) protein. SUMO-modified forms of PML are essential for the recruitment of Death domain-associated protein 6 (DAXX) to PML nuclear bodies.

Participants:
(1) Protein PML (PML)
(2) SUMO-conjugating enzyme UBC9 (UBE2I)
(3) Death domain-associated protein 6 (DAXX)
(4) Small ubiquitin-related modifier 2 (SUMO2)

Interactions

This is a sequential switch. The interactions in this switch take place in a defined order. The order is defined by numbers to the left of each interaction. More than one interaction can occur in each step, these interactions occur independently but are necessary for future step

1Interaction #1 PML - UBE2I

Interfaces
(1) MOD_SUMO motif (₁₅₉LKHE₁₆₂) in Protein PML (PML)
(2) Ubiquitin-conjugating enzyme_(8-152) in SUMO-conjugating enzyme UBC9 (UBE2I)

Interaction Regulation
PTM-dependent Induction (MOD:01149 at K₁₆₀ on Protein PML (PML)) of the Protein PML (PML) MOD_SUMO motif - SUMO-conjugating enzyme UBC9 (UBE2I) Ubiquitin-conjugating enzyme interaction

2Interaction #2 DAXX - SUMO2

Interfaces
(3) LIG_SUMO_SBM_1 motif (₇₃₃IIVLSDSD₇₄₀) in Death domain-associated protein 6 (DAXX)
(4) Ubiquitin-2 like Rad60 SUMO-like_(18-88) in Small ubiquitin-related modifier 2 (SUMO2)

Interaction Regulation
PTM-dependent Induction (MOD:01149 at K₁₆₀ on ) of the Death domain-associated protein 6 (DAXX) LIG_SUMO_SBM_1 motif - Small ubiquitin-related modifier 2 (SUMO2) Ubiquitin-2 like Rad60 SUMO-like interaction

Context

Switch localisation
ELM curated: nucleus, PML body

Inferred: cytoplasm, PML body, nucleolus, cytosol, nucleus

Switch pathways

Inferred: Ubiquitin mediated proteolysis, RNA transport

References

(1) An Acetylation Switch Regulates SUMO-Dependent Protein Interaction Networks.
Ullmann et al. Mol. Cell (2012)