Switches.ELM

Switch #:

SWTI000676

Switch type:
Binary

Switch subtype:
Physicochemical compatibility

Description:
Phosphorylation of T387 adjacent to the clathrin-binding motif of Amphiphysin (AMPH) by CK2 subfamily inhibits binding to the Clathrin heavy chain 1 (CLTC). A second clathrin-binding motif in Amphiphysin (AMPH) is regulated in a similar manner (see switch details). Both these motifs cooperate in avidity-based binding to Clathrin heavy chain 1 (CLTC) (see switch details).

Participants:
(1) Amphiphysin (AMPH)
(2) Clathrin heavy chain 1 (CLTC)

Interactions

Interaction #1 AMPH - CLTC

Interfaces
(1) LIG_Clathr_ClatBox_2 motif (₃₈₀LPWDLW₃₈₅) in Amphiphysin (AMPH)
(2) Clathrin propeller repeat_(19-56) and _(148-187) and _(198-234) in Clathrin heavy chain 1 (CLTC)

Interaction Regulation
PTM-dependent Inhibition (Phosphorylation of T₃₈₇ on Amphiphysin (AMPH)) of the Amphiphysin (AMPH) LIG_Clathr_ClatBox_2 motif - Clathrin heavy chain 1 (CLTC) Clathrin propeller repeat interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: T₃₈₇: CK2 subfamily

Inferred Regulatory Enzymes for switch
Putative modifying enzymes for residue: T₃₈₇ : Casein kinase II subunit alpha (CSNK2A1).

Context

References

(1) Nerve growth factor-induced phosphorylation of amphiphysin-1 by casein kinase 2 regulates clathrin-amphiphysin interactions.
Döring et al. J. Neurochem. (2006)