Switches.ELM

Switch #:

SWTI000005

Switch type:
Binary

Switch subtype:
Physicochemical compatibility

Description:
Phosphorylation of T130 in the PLK-docking motif of M-phase inducer phosphatase 3 (CDC25C) by Cyclin-dependent kinase 1 (CDK1)-Cyclin AB subfamily generates a recruitment site for Serine/threonine-protein kinase PLK1 (PLK1), which then phosphorylates M-phase inducer phosphatase 3 (CDC25C). This results in inactivation of the NES of M-phase inducer phosphatase 3 (CDC25C), thereby promoting its nuclear localization.

Participants:
(1) M-phase inducer phosphatase 3 (CDC25C)
(2) Serine/threonine-protein kinase PLK1 (PLK1)

Interactions

Interaction #1 CDC25C - PLK1

Interfaces
(1) ELM:LIG_PLK motif (₁₂₉STP₁₃₁) in M-phase inducer phosphatase 3 (CDC25C)
(2) POLO box duplicated region_(417-584) in Serine/threonine-protein kinase PLK1 (PLK1)

Interaction Regulation
PTM-dependent Induction (Phosphorylation of T₁₃₀ on M-phase inducer phosphatase 3 (CDC25C)) of the M-phase inducer phosphatase 3 (CDC25C) LIG_PLK motif - Serine/threonine-protein kinase PLK1 (PLK1) POLO box duplicated region interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: T₁₃₀: Cyclin-dependent kinase 1 (CDK1)

Inferred Regulatory Enzymes for switch
Putative modifying enzymes for residue: T₁₃₀ : Cyclin-dependent kinase 1 (CDK1).

Context

Switch localisation
Curated: nucleus

References

(1) Proteomic screen finds pSer/pThr-binding domain localizing Plk1 to mitotic substrates.
Elia et al. Science (2003)