Switches.ELM

Switch #:

SWTI000014

Switch type:
Binary

Switch subtype:
Physicochemical compatibility

Description:
Phosphorylation of Y55 in Protein sprouty homolog 2 (SPRY2) is necessary for binding to the TKB domain of E3 ubiquitin-protein ligase CBL (CBL).

Participants:
(1) Protein sprouty homolog 2 (SPRY2)
(2) E3 ubiquitin-protein ligase CBL (CBL)

Interactions

Interaction #1 SPRY2 - CBL

Interfaces
(1) ELM:LIG_TKB motif (₅₅YTEGPT₆₀) in Protein sprouty homolog 2 (SPRY2)
(2) E3 ubiquitin-protein ligase CBL (CBL)

Interaction Regulation
PTM-dependent Induction (Phosphorylation of Y₅₅ on Protein sprouty homolog 2 (SPRY2)) of the Protein sprouty homolog 2 (SPRY2) LIG_TKB motif - E3 ubiquitin-protein ligase CBL (CBL) interaction

Inferred Regulatory Enzymes for switch
Putative modifying enzymes for residue: Y₅₅ : Proto-oncogene tyrosine-protein kinase Src (SRC).

Additional Information
Affinity : pY55: 0.31 µM
Structural information: 3OB1

Context

Switch localisation

Switch pathways

References

(1) Additional serine/threonine phosphorylation reduces binding affinity but preserves interface topography of substrate proteins to the c-Cbl TKB domain.
Sun et al. PLoS ONE (2010)