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Physicochemical compatibility

Phosphorylation of S140 in the BRCT-binding motif of Histone H2A.x (H2AFX) induces binding to the Mediator of DNA damage checkpoint protein 1 (MDC1) protein.

(1) Histone H2A.x (H2AFX)
(2) Mediator of DNA damage checkpoint protein 1 (MDC1)

Interaction #1 H2AFX - MDC1

(1) LIG_BRCT_MDC1_1 motif (139ASQEY143) in Histone H2A.x (H2AFX)
(2) BRCA1 C Terminus (BRCT) domain (1883-1960) and (1993-2072) in Mediator of DNA damage checkpoint protein 1 (MDC1)

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S140 on Histone H2A.x (H2AFX)) of the Histone H2A.x (H2AFX) LIG_BRCT_MDC1_1 motif - Mediator of DNA damage checkpoint protein 1 (MDC1) BRCA1 C Terminus (BRCT) domain interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: S140: Serine-protein kinase ATM (ATM),DNA-dependent protein kinase catalytic subunit (PRKDC)

Additional Information
Structural information: 2AZM

(1) MDC1 directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks.
Stucki et al. Cell (2005)

(2) ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation.
Stiff et al. Cancer Res. (2004)

See also

Other switches involving interfaces
BRCA1 C Terminus (BRCT) domain - 6 more (view)

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