Switches.ELM

Switch #:

SWTI000209

Switch type:
Binary

Switch subtype:
Physicochemical compatibility

Description:
Phosphorylation of T1269 in the PDZ-binding motif of Breakpoint cluster region protein (BCR) inhibits its interaction with the Afadin (MLLT4) protein.

Participants:
(1) Breakpoint cluster region protein (BCR)
(2) Afadin (MLLT4)

Interactions

Interaction #1 BCR - MLLT4

Interfaces
(1) LIG_PDZ_Class_1 motif (₁₂₆₆LFSTEV₁₂₇₁) in Breakpoint cluster region protein (BCR)
(2) PDZ domain (Also known as DHR or GLGF)_(1008-1090) in Afadin (MLLT4)

Interaction Regulation
PTM-dependent Abrogation (Phosphorylation of T₁₂₆₉ on Breakpoint cluster region protein (BCR)) of the Breakpoint cluster region protein (BCR) LIG_PDZ_Class_1 motif - Afadin (MLLT4) PDZ domain (Also known as DHR or GLGF) interaction

Additional Information
Affinity : BCR (Wild type): 85.9 µM, BCR (T1269D mutant): >1000 µM
Structural information: 2AIN

Context

References

(1) Solution structure and backbone dynamics of the AF-6 PDZ domain/Bcr peptide complex.
Chen et al. Protein Sci. (2007)

(2) Characterization of a putative phosphorylation switch: adaptation of SPOT synthesis to analyze PDZ domain regulation mechanisms.
Boisguerin et al. Chembiochem (2007)