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Switch #:
SWTI000212
Switch type:
Binary
Switch subtype:
Allostery

Description:
Binding of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to the AP-2 complex alpha, beta and mu subunits exposes a binding site on the AP-2 complex subunit mu (Ap2m1) subunit for recruitment of Trans-Golgi network integral membrane protein TGN38 (Ttgn1) via an endocytosis motif.

Participants:
(1) Trans-Golgi network integral membrane protein TGN38 (Ttgn1)
(2) AP-2 complex subunit mu (Ap2m1)

Interactions
Interaction #1 Ttgn1 - Ap2m1

Interfaces
(1) TRG_ENDOCYTIC_2 motif (350YQRL353) in Trans-Golgi network integral membrane protein TGN38 (Ttgn1)
(2) Adaptor complexes medium subunit family (1-137) in AP-2 complex subunit mu (Ap2m1)

Interaction Regulation
Effector binding Induction (1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate) of the Trans-Golgi network integral membrane protein TGN38 (Ttgn1) TRG_ENDOCYTIC_2 motif - AP-2 complex subunit mu (Ap2m1) Adaptor complexes medium subunit family interaction

Additional Information
Affinity : Isolated C-mu2: 1.9 µM, AP2 core in the presence of heparin: 3.4 µM, AP2 core in the absence of heparin: 4000 µM
Structural information: 2XA7, 1BXX
Context
Switch localisation
ELM curated: cytosol, clathrin-coated endocytic vesicle, plasma membrane
References

(1) A large-scale conformational change couples membrane recruitment to cargo binding in the AP2 clathrin adaptor complex.
Jackson et al. Cell (2010)

See also

Other switches involving interfaces
TRG_ENDOCYTIC_2 - 21 more (view)
Adaptor complexes medium subunit family - 21 more (view)


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