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Switch #:
SWTI000297
Switch type:
Specificity
Switch subtype:
Altered binding specificity

Description:
Phosphorylation of Zinc finger protein SNAI1 (SNAI1) at S100 generates a docking site for Glycogen synthase kinase-3 beta (GSK3B). Subsequent phosphorylation of S96 by GSK3B targets Zinc finger protein SNAI1 (SNAI1) to the SCF ubiquitin ligase complexes F-box/WD repeat-containing protein 1A (BTRC), which marks it for degradation.

Participants:
(1) Zinc finger protein SNAI1 (SNAI1)
(2) Glycogen synthase kinase-3 beta (GSK3B)
(3) F-box/WD repeat-containing protein 1A (BTRC)

Interactions
This is a sequential switch. The interactions in this switch take place in a defined order. The order is defined by numbers to the left of each interaction. More than one interaction can occur in each step, these interactions occur independently but are necessary for future step
1Interaction #1 SNAI1 - GSK3B

Is mutually exclusive with Interaction #2 SNAI1 - BTRC

Interfaces
(1) MOD_GSK3_1 motif (93DEDSGKGS100) in Zinc finger protein SNAI1 (SNAI1)
(2) Protein kinase domain (56-340) in Glycogen synthase kinase-3 beta (GSK3B)

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S100 on Zinc finger protein SNAI1 (SNAI1)) of the Zinc finger protein SNAI1 (SNAI1) MOD_GSK3_1 motif - Glycogen synthase kinase-3 beta (GSK3B) Protein kinase domain interaction


2Interaction #2 SNAI1 - BTRC

Requires Interaction #1 SNAI1 - GSK3B
Is mutually exclusive with Interaction #1 SNAI1 - GSK3B

Interfaces
(3) DEG_SCF_TRCP1_1 motif (95DSGKGS100) in Zinc finger protein SNAI1 (SNAI1)
(4) WD domain, G-beta repeat (297-329) and (333-369) and (373-409) and (416-452) and (456-492) and (496-532) and (545-581) in F-box/WD repeat-containing protein 1A (BTRC)

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S96 and S100 on Zinc finger protein SNAI1 (SNAI1)) of the Zinc finger protein SNAI1 (SNAI1) DEG_SCF_TRCP1_1 motif - F-box/WD repeat-containing protein 1A (BTRC) WD domain, G-beta repeat interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: S96: Glycogen synthase kinase-3 beta (GSK3B)


Context
References

(1) The hypoxia-controlled FBXL14 ubiquitin ligase targets SNAIL1 for proteasome degradation.
ViƱas-Castells et al. J. Biol. Chem. (2010)

(2) Dual regulation of Snail by GSK-3beta-mediated phosphorylation in control of epithelial-mesenchymal transition.
Zhou et al. Nat. Cell Biol. (2004)

See also

Other switches involving participants
Glycogen synthase kinase-3 beta (GSK3B) - 18 more (view)
F-box/WD repeat-containing protein 1A (BTRC) - 17 more (view)

Other switches involving interfaces
Protein kinase domain - 35 more (view)
MOD_GSK3_1 - 18 more (view)
WD domain, G-beta repeat - 36 more (view)
DEG_SCF_TRCP1_1 - 17 more (view)




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