Switches.ELM

Switch #:

SWTI000326

Switch type:
Specificity

Switch subtype:
Domain hiding

Description:
Binding of meiRNA meiotic non-coding RNA (meiRNA) to the RRM domains of Meiosis protein mei2 (mei2) is essential for promotion of premeiotic DNA synthesis and meiosis I and is blocked by Pat1-mediated phosphorylation-induced binding of the 14-3-3 protein DNA damage checkpoint protein rad24 (rad24) to 2 14-3-3 binding motifs in mei2.

Participants:
(1) Meiosis protein mei2 (mei2)
(2) DNA damage checkpoint protein rad24 (rad24)
(3) meiotic non-coding RNA (meiRNA)

Interactions

Interaction #1 mei2 - rad24

Is mutually exclusive with Interaction #2 - mei2

Interfaces
(1) LIG_14-3-3_1 and LIG_14-3-3_2 motif (₄₃₅RTESSP₄₄₀ and ₅₂₃RRSLTVG₅₂₉) in Meiosis protein mei2 (mei2)
(2) 14-3-3 protein_(6-241) in DNA damage checkpoint protein rad24 (rad24)

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S₃₃₈ and T₅₂₇ on Meiosis protein mei2 (mei2)) of the Meiosis protein mei2 (mei2) LIG_14-3-3_1;ELM motif - DNA damage checkpoint protein rad24 (rad24) 14-3-3 protein interaction

Interaction #2 - mei2

Is mutually exclusive with Interaction #1 mei2 - rad24

Interfaces
(3) meiotic non-coding RNA (meiRNA)
(4) RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)_(197-264) and RNA recognition motif 2_(596-692) in Meiosis protein mei2 (mei2)

Interaction Regulation
Effector binding Abrogation (DNA damage checkpoint protein rad24 (rad24)) of the meiotic non-coding RNA (meiRNA) motif - Meiosis protein mei2 (mei2) interaction

Context

Switch localisation

References

(1) 14-3-3 protein interferes with the binding of RNA to the phosphorylated form of fission yeast meiotic regulator Mei2p.
Sato et al. Curr. Biol. (2002)