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Switch #:
SWTI000357
Switch type:
Avidity-sensing

Description:
Phosphorylation of two 14-3-3-binding motifs in Kinase suppressor of Ras 1 (Ksr1) by Q03141 induces high-avidity binding to dimeric 14-3-3 protein beta/alpha (Ywhab). This interaction prevents Kinase suppressor of Ras 1 (Ksr1) to localise to the membrane where it is involved in activation of MAP kinases by Q99N57 in response to growth factors.

Participants:
(1) 14-3-3 protein beta/alpha (Ywhab)
(2) Kinase suppressor of Ras 1 (Ksr1)

Interactions
This is a sequential switch. The interactions in this switch take place in a defined order. The order is defined by numbers to the left of each interaction. More than one interaction can occur in each step, these interactions occur independently but are necessary for future step
2Interaction #2 Ksr1 - Interaction #1 Ywhab - Ywhab

Requires Interaction #1 Ywhab - Ywhab

Interfaces
(3) LIG_14-3-3_3 motif (294RSKSHE299) in Kinase suppressor of Ras 1 (Ksr1)
(4) Interaction #1 Ywhab - Ywhab

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S297 on Kinase suppressor of Ras 1 (Ksr1)) of the Kinase suppressor of Ras 1 (Ksr1) LIG_14-3-3_3 motif - 14-3-3 protein interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: S297: MAP/microtubule affinity-regulating kinase 3 (Mark3)

2Interaction #3 Ksr1 - Interaction #1 Ywhab - Ywhab

Requires Interaction #1 Ywhab - Ywhab

Interfaces
(5) LIG_14-3-3_3 motif (389RTESVP394) in Kinase suppressor of Ras 1 (Ksr1)
(6) Interaction #1 Ywhab - Ywhab

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S392 on Kinase suppressor of Ras 1 (Ksr1)) of the Kinase suppressor of Ras 1 (Ksr1) LIG_14-3-3_3 motif - 14-3-3 protein interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: S392: MAP/microtubule affinity-regulating kinase 3 (Mark3)


Context
References

(1) Identification of constitutive and ras-inducible phosphorylation sites of KSR: implications for 14-3-3 binding, mitogen-activated protein kinase binding, and KSR overexpression.
Cacace et al. Mol. Cell. Biol. (1999)

(2) C-TAK1 regulates Ras signaling by phosphorylating the MAPK scaffold, KSR1.
Müller et al. Mol. Cell (2001)

See also

Other switches involving participants
14-3-3 protein beta/alpha (Ywhab) - 1 more (view)

Other switches involving interfaces
14-3-3 protein - 38 more (view)
LIG_14-3-3_3 - 16 more (view)




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