Switch #: SWTI000358

Switch type: Avidity-sensing

Description:
Phosphorylation of two 14-3-3-binding motifs in Slowpoke-binding protein (Slob) by Calcium/calmodulin-dependent protein kinase type II alpha chain (CaMKII) induces high-avidity binding to dimeric 14-3-3 protein zeta (14-3-3zeta). This interaction recruits 14-3-3 protein zeta (14-3-3zeta) to Calcium-activated potassium channel slowpoke (slo) in the presynapse of neuromuscular junctions.

Participants:
(1) 14-3-3 protein zeta (14-3-3zeta)
(2) Slowpoke-binding protein (Slob)

This is a sequential switch. The interactions in this switch take place in a defined order. The order is defined by numbers to the left of each interaction. More than one interaction can occur in each step, these interactions occur independently but are necessary for future step

1Interaction #1 14-3-3zeta - 14-3-3zeta

Interfaces
(1) 14-3-3 protein in 14-3-3 protein zeta (14-3-3zeta)
(2) 14-3-3 protein _(6-239) in 14-3-3 protein zeta (14-3-3zeta)

2Interaction #2 Slob - Interaction #1 14-3-3zeta - 14-3-3zeta

Requires Interaction #1 14-3-3zeta - 14-3-3zeta

Interfaces
(3) LIG_14-3-3_3 motif (₅₁RSNSAI₅₆) in Slowpoke-binding protein (Slob)
(4) Interaction #1 14-3-3zeta - 14-3-3zeta

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S₅₄ on Slowpoke-binding protein (Slob)) of the Slowpoke-binding protein (Slob) LIG_14-3-3_3 motif - 14-3-3 protein interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: S₅₄: Calcium/calmodulin-dependent protein kinase type II alpha chain (CaMKII)

2Interaction #3 Slob - Interaction #1 14-3-3zeta - 14-3-3zeta

Requires Interaction #1 14-3-3zeta - 14-3-3zeta

Interfaces
(5) LIG_14-3-3_3 motif (₇₆RSASSE₈₁) in Slowpoke-binding protein (Slob)
(6) Interaction #1 14-3-3zeta - 14-3-3zeta

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S₇₉ on Slowpoke-binding protein (Slob)) of the Slowpoke-binding protein (Slob) LIG_14-3-3_3 motif - 14-3-3 protein interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: S₇₉: Calcium/calmodulin-dependent protein kinase type II alpha chain (CaMKII)

(1) A dynamically regulated 14-3-3, Slob, and Slowpoke potassium channel complex in Drosophila presynaptic nerve terminals.
Zhou et al. Neuron (1999)

Other switches involving participants
Slowpoke-binding protein (Slob) - 1 more (view)

Other switches involving interfaces
14-3-3 protein - 38 more (view)
LIG_14-3-3_3 - 16 more (view)