Switch #: SWTI000459

Switch type: Avidity-sensing

Description:
Phosphorylation of two 14-3-3-binding motifs in Meiosis protein mei2 (mei2) by Negative regulator of sexual conjugation and meiosis (ran1) induces high-avidity binding to dimeric DNA damage checkpoint protein rad24 (rad24), with pT527 being the high-affinity interaction site.

Participants:
(1) DNA damage checkpoint protein rad24 (rad24)
(2) Meiosis protein mei2 (mei2)

This is a sequential switch. The interactions in this switch take place in a defined order. The order is defined by numbers to the left of each interaction. More than one interaction can occur in each step, these interactions occur independently but are necessary for future step

1Interaction #1 rad24 - rad24

Interfaces
(1) 14-3-3 protein in DNA damage checkpoint protein rad24 (rad24)
(2) 14-3-3 protein _(6-241) in DNA damage checkpoint protein rad24 (rad24)

2Interaction #2 mei2 - Interaction #1 rad24 - rad24

Requires Interaction #1 rad24 - rad24

Interfaces
(3) LIG_14-3-3_1 motif (₄₃₅RTESSP₄₄₀) in Meiosis protein mei2 (mei2)
(4) Interaction #1 rad24 - rad24

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S₄₃₈ on Meiosis protein mei2 (mei2)) of the Meiosis protein mei2 (mei2) LIG_14-3-3_1 motif - 14-3-3 protein interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: S₄₃₈: Negative regulator of sexual conjugation and meiosis (ran1)

2Interaction #3 mei2 - Interaction #1 rad24 - rad24

Requires Interaction #1 rad24 - rad24

Interfaces
(5) LIG_14-3-3_2 motif (₅₂₃RRSLTVG₅₂₉) in Meiosis protein mei2 (mei2)
(6) Interaction #1 rad24 - rad24

Interaction Regulation
PTM-dependent Induction (Phosphorylation of T₅₂₇ on Meiosis protein mei2 (mei2)) of the Meiosis protein mei2 (mei2) LIG_14-3-3_2 motif - 14-3-3 protein interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: T₅₂₇: Negative regulator of sexual conjugation and meiosis (ran1)

(1) 14-3-3 protein interferes with the binding of RNA to the phosphorylated form of fission yeast meiotic regulator Mei2p.
Sato et al. Curr. Biol. (2002)

(2) The 14-3-3 proteins Rad24 and Rad25 negatively regulate Byr2 by affecting its localization in Schizosaccharomyces pombe.
Ozoe et al. Mol. Cell. Biol. (2002)

Other switches involving participants
DNA damage checkpoint protein rad24 (rad24) - 1 more (view)
Meiosis protein mei2 (mei2) - 1 more (view)

Other switches involving interfaces
14-3-3 protein - 38 more (view)
LIG_14-3-3_1 - 13 more (view)
LIG_14-3-3_2 - 7 more (view)