Switches.ELM

Switch #:

SWTI000576

Switch type:
Binary

Description:
Alternative splicing removes the SUMO motif of Protein PML (PML), abrogating binding to SUMO-conjugating enzyme UBC9 (UBE2I). The study identified a major sumoylation site within the nuclear localisation signal (NLS) of PML. Although they did not determine whether the lysine residue regulates the NLS, they found that sumoylation was not necessary for nuclear localisation and that SUMO-modification only occurs in the nucleus.

Participants:
(1) Protein PML (PML)
(2) SUMO-conjugating enzyme UBC9 (UBE2I)

Interactions

Interaction #1 PML - UBE2I

Interfaces
(1) MOD_SUMO motif (₄₈₉IKME₄₉₂) in Protein PML (PML)
(2) Ubiquitin-conjugating enzyme_(8-152) in SUMO-conjugating enzyme UBC9 (UBE2I)

Interaction Regulation
Alternative splicing Abrogation of the Protein PML (PML) MOD_SUMO motif - SUMO-conjugating enzyme UBC9 (UBE2I) Ubiquitin-conjugating enzyme interaction

Context

Switch localisation
ELM curated: nucleus, PML body

Inferred: cytoplasm, PML body

Switch pathways

Inferred: Ubiquitin mediated proteolysis

References

(1) Alternative splicing of PML transcripts predicts coexpression of several carboxy-terminally different protein isoforms.
Fagioli et al. Oncogene (1992)