Switches.ELM

Switch #:

SWTI000578

Switch type:
Binary

Description:
Alternative splicing removes the di-leucine endocytosis motif of Prolactin receptor (Prlr), partially inhibiting binding to AP-2 complex subunit sigma (AP2S1) and the rate of endocytosis. Isoform Long of Prolactin receptor (Prlr) (also known as PRLR-long) has a longer C-terminal tail than the Isoform Short of Prolactin receptor (Prlr) (also known as PRLR-short) splice variant. PRLR-long internalises faster than PRLR-short due to two additional di-leucine motifs in the C-terminus (where the adjacent phenylalanine also plays a role). PRLR-short has two di-leucine motifs whereas PRLR-long has four.

Participants:
(1) Prolactin receptor (Prlr)
(2) AP-2 complex subunit sigma (AP2S1)

Interactions

Interaction #1 Prlr - AP2S1

Interfaces
(1) TRG_LysEnd_APsAcLL_1 motif (₃₀₁DCEDLL₃₀₆) in Prolactin receptor (Prlr)
(2) Clathrin adaptor complex small chain_(1-142) in AP-2 complex subunit sigma (AP2S1)

Interaction Regulation
Alternative splicing Abrogation of the Prolactin receptor (Prlr) TRG_LysEnd_APsAcLL_1 motif - AP-2 complex subunit sigma (AP2S1) Clathrin adaptor complex small chain interaction

Context

Switch localisation
ELM curated: Endocytic vesicle, cytosol

References

(1) Multiple internalization motifs differentially used by prolactin receptor isoforms mediate similar endocytic pathways.
Lu et al. Mol. Endocrinol. (2002)