Switch #: SWTI000580

Switch type: Binary

Switch subtype: Pre-translational

Description:
Alternative splicing removes the nuclear localisation signal (NLS) of Breast cancer type 1 susceptibility protein (BRCA1), abrogating binding to Importin subunit alpha-1 (KPNA1) and import into the nucleus. The study compared the full-length Brca1 splice variant (Isoform 1 of Breast cancer type 1 susceptibility protein (BRCA1)) to the Delta11b isoform (Isoform Delta11b of Breast cancer type 1 susceptibility protein (BRCA1)). The shorter isoform is missing exon 11b and differs in a number of ways. Firstly, it lacks an NLS and therefore has a cytoplasmic localisation. Also, when over-expressed, the Delta11b isoform was not toxic, suggesting nuclear localisation is important for Brca1's toxic behaviour.

Participants:
(1) Breast cancer type 1 susceptibility protein (BRCA1)
(2) Importin subunit alpha-1 (KPNA1)

(1) Differential subcellular localization, expression and biological toxicity of BRCA1 and the splice variant BRCA1-delta11b.
Wilson et al. Oncogene (1997)

(2) Emerging roles of BRCA1 alternative splicing.
Orban et al. MP, Mol. Pathol. (2003)

Other switches involving participants
Breast cancer type 1 susceptibility protein (BRCA1) - 6 more (view)
Importin subunit alpha-1 (KPNA1) - 9 more (view)

Other switches involving interfaces
TRG_NLS_MonoExtN_4 - 10 more (view)
Armadillo/beta-catenin-like repeat - 16 more (view)