Switch #: SWTI000594

Switch type: Binary

Switch subtype: Pre-translational

Description:
Alternative splicing removes the PDZ-binding motif of Pleckstrin homology domain-containing family G member 5 (Plekhg5), abrogating binding to PDZ domain-containing protein GIPC1 (Gipc1). The PDZ adaptor protein Gipc1 (Synectin) bound the longer splice variant, Isoform SYX1 of Pleckstrin homology domain-containing family G member 5 (Plekhg5) (Syx1), which was targeted to the plasma membrane in a Synectin-dependent manner. The shorter variant, Isoform SYX2 of Pleckstrin homology domain-containing family G member 5 (Plekhg5) (Syx2), was diffusely distributed in the cytoplasm. Expression of Syx1 augmented endothelial cell migration and tube formation, whereas Syx2 expression did not. Significant expression of Syx2 was only seen in brain tumour cells, which also exhibited high basal Transforming protein RhoA (Rhoa) activity.

Participants:
(1) Pleckstrin homology domain-containing family G member 5 (Plekhg5)
(2) PDZ domain-containing protein GIPC1 (Gipc1)

(1) A PDZ-binding motif as a critical determinant of Rho guanine exchange factor function and cell phenotype.
Liu et al. Mol. Biol. Cell (2006)

Other switches involving participants
PDZ domain-containing protein GIPC1 (Gipc1) - 1 more (view)

Other switches involving interfaces
PDZ domain (Also known as DHR or GLGF) - 45 more (view)
LIG_PDZ_Class_1 - 41 more (view)